This indicated that spoVG was located upstream of abrB and sinR in the regulatory pathway of B. The phenotypes of these double mutants were congruent with those of the single abrB and sinR deletion strains, respectively, which showed increased biofilm formation. The role of SpoVG in regulating biofilm formation was further explored by deleting the genes abrB and sinR in the Δ spoVG mutant, respectively, generating the double mutant strains Δ spoVGΔ abrB and Δ spoVGΔ sinR. The transcription levels of two genes closely related to biofilm-formation, sipW and calY, were downregulated in a Δ spoVG mutant. In addition, SpoVG could influence biofilm formation of B. cereus 0–9, which is identical to that of Bacillus subtilis but differs from that of Bacillus anthracis. cereus 0–9 in germination medium, SpoVG is crucial for regulating sporulation stage V of B. ResultsĪccording to observation of the sporulation process of B. ![]() cereus 0–9 and its mutants were determined to study the functions of the target genes, and the transcription level of the target gene was determined by qRT-PCR. The biofilm production and colonial morphology of B. Transcriptional fusion strains were constructed by overlapping PCR technique, and the promoter activity of the target gene was detected by measuring its fluorescence intensity. We further determined the spore yields and biofilm formation abilities of test strains. cereus 0–9 and its mutants were observed by fluorescence staining method. The gene knockout strains were constructed by homologous recombination, and the sporulation process of B. This study aimed to explore the multiple functions of SpoVG in B. SpoVG, a regulator that is broadly conserved among many Gram-positive bacteria, may help this organism coordinate environmental growth and virulence to survive. doi: 10.1073/pnas.Bacillus cereus 0–9, a Gram-positive, endospore-forming bacterium isolated from healthy wheat roots in our previous research, is considered to be an effective biocontrol strain against several soil-borne plant diseases. Wang X, Montero Llopis P, Rudner DZ (2014) Bacillus subtilis chromosome organization oscillates between two distinct patterns. Grossman AD, Losick R (1988) Extracellular control of spore formation in Bacillus subtilis. Harwood CR, Cutting SM (1990) Molecular biological methods for Bacillus. doi: 10.1016/j.cub.2010.04.045īalaban NQ, Merrin J, Chait R, Kowalik L, Leibler S (2004) Bacterial persistence as a phenotypic switch. ![]() Wang P, Robert L, Pelletier J, Dang WL, Taddei F, Wright A, Jun S (2010) Robust growth of Escherichia coli. Norman TM, Lord ND, Paulsson J, Losick R (2013) Memory and modularity in cell-fate decision making. doi: 10.1101/cshperspect.a000307įornasiero EF, Opazo F (2015) Super-resolution imaging for cell biologists: concepts, applications, current challenges and developments. Cold Spring Harb Perspect Biol 2(4):a000307. Rudner DZ, Losick R (2010) Protein subcellular localization in bacteria. Paredes-Sabja D, Setlow P, Sarker MR (2011) Germination of spores of Bacillales and Clostridiales species: mechanisms and proteins involved. Setlow P (2014) Spore resistance properties. Tan IS, Ramamurthi KS (2014) Spore formation in Bacillus subtilis.
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |